Comprehensive Identification and Profiling of miRNAs Involved in Terpenoid Synthesis of Gleditsia sinensis Lam.

Gleditsia sinensis Lam. is a tree with worldwide distribution and important economic medicinal values; its pods contain terpenoids including gleditsioside, thiamine, brassinosteroids. However, thus far, there are few studies on the terpenoid regulation of G. at molecular level. microRNA (miRNA) class small RNAs conserved crucial roles in diverse biological processes during plant growth development. To identify miRNAs evaluate their involvement synthesis, this investigation quantified content changes saponins three developmental stages: May (pod-setting stage), July (elongation September (browning then we performed genome-wide miRNA profiles development stages pods. A total 351 belonging to 216 families were identified, among which 36 exist specifically legumes. Through target analysis, 708 unigenes predicted be candidate targets 37 differentially expressed miRNAs. The miR838-3p miR2093-5p involved derived branches monoterpenes brassinosteroid biosynthesis (BRB), indole alkaloid (IAB). Intriguingly, miR829-3p.1 take part thiamine biosynthesis, miR4414b miR5037a main process cytokinin synthesis. corresponding participated BRB, IAB, backbone enriched significantly, suggesting that miR2093-5p, miR4414b, miR5037a, miR829-3p.1, play indispensable triterpenoid saponin monoterpenoid biosynthesis. date, first report identification expression different pods, provides basis for further uncovering synthesis new insights into role